Study of Antimicrobial Activity of Bruguiera Cylindrica Leaf Extract Against Escherichia Coli and Staphylococcus aureus

Almasyhur, Farida and Audah, Kholis Abdurachim and Susanto, Hery (2016) Study of Antimicrobial Activity of Bruguiera Cylindrica Leaf Extract Against Escherichia Coli and Staphylococcus aureus. Bachelor thesis, Swiss German University.

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Abstract

The aim of this study is to discover the antimicrobial activity of Bruguiera cylindrica leaf extract using three different solvent based on the level of polarity against two different grams of bacteria, Escherichia coli and Staphylococcus aureus. Zone of inhibition determination using agar-disk diffusion has been studied. Two different concentrations of each solvent - ethanol, water, and hexane, has been also studied. The result showed that the highest diameter of zone of inhibition against both bacteria was ethanol extract, which significantly yielded 14.30 mm diameter of zone of inhibition against Staphylococcus aureus and 13.30 mm diameter of zone of inhibition against Escherichia coli. Along with the result of inhibition zone determination, highest concentration of flavonoid and phenolic content yielded by ethanol extract with flavonoid compound concentration of 979.49 ± 28.32 mg QCE/100 g dry mass and total phenolic compound concentration of 1877.97 ± 39.88 mg GAE/100 g dry mass. In conclusion, ethanol extract was very effective to establish antimicrobial activity against multidrug bacteria.

Item Type: Thesis (Bachelor)
Uncontrolled Keywords: Bruguiera Cylindrica; Multidrug-resistant Bacteria; Antimicrobial Activity; Flavonoid; Total Phenolic Content.
Subjects: R Medicine > R Medicine (General) > R856 Biomedical engineering
R Medicine > RM Therapeutics. Pharmacology > RM267 Antibiotics--Research
Divisions: Faculty of Life Sciences and Technology > Department of Biomedical Engineering
Depositing User: Atroridho Rizky
Date Deposited: 04 Nov 2020 02:54
Last Modified: 04 Nov 2020 02:54
URI: http://repository.sgu.ac.id/id/eprint/1381

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