Anthika, Bea and Kusumocahyo, Samuel P. and Sutanto, Hery (2015) Ultrasonic Approach in Butterfly Pea Extraction and Extract Sterilization by Ultrafiltration for Eye Drop Active Ingredient. Bachelor thesis, Swiss German University.

Preview	Text Bea Anthika 14111003 TOC.pdf Download (361kB) | Preview
	Text Bea Anthika 14111003 1.pdf Restricted to Registered users only Download (321kB)
	Text Bea Anthika 14111003 2.pdf Restricted to Registered users only Download (595kB)
	Text Bea Anthika 14111003 3.pdf Restricted to Registered users only Download (635kB)
	Text Bea Anthika 14111003 4.pdf Restricted to Registered users only Download (1MB)
	Text Bea Anthika 14111003 5.pdf Restricted to Registered users only Download (196kB)
Preview	Text Bea Anthika 14111003 Ref.pdf Download (323kB) | Preview

Abstract

The aim of this study is to evaluate butterfly pea ultrasound-assisted extraction (UAE) and sterilize the extract using ultrafiltration (UF) membrane for use as an active ingredient in phytopharmaceutical eye drops. The effect of petal-to-leaf (PTL) ratio and extraction time on anthocyanin concentration and antibacterial activity has been studied. The result showed that the optimum configuration was PTL ratio of 1:0 and extraction time of 30 minutes, which significantly yielded anthocyanin concentration of 35.41 ± 0.62 mg/l and exhibited the highest antibacterial activity against S.aureus in terms of inhibition zone of 14.75 ± 1.06 mm. Subsequently, feasibility of using UF membrane to sterilize the butterfly pea extract was also studied. Number of bacteria in the extract was varied to check whether UF was able to sterilize the extract regardless of the degree of bacterial contamination. The results showed no presence of bacteria in permeate during 21 days of observation and an increase in anthocyanin concentration as well as antibacterial activity of the extract. In conclusion, UF was very effective to sterilize butterfly pea extract without the application of heat, thus, preserve the heat- sensitive compounds such as anthocyanins.

Item Type:	Thesis (Bachelor)
Uncontrolled Keywords:	Butterfly Pea; Ultrasound-assisted Extraction (UAE); Sterilization; Ultrafiltration (UF) Membrane; Antibacterial Activity; Anthocyanins
Subjects:	H Social Sciences > HD Industries. Land use. Labor > HD9665 Pharmaceutical Industry Q Science > QC Physics > QC244 Ultrasonic waves R Medicine > RS Pharmacy and materia medica > RS192 Pharmaceutical technology
Divisions:	Faculty of Life Sciences and Technology > Department of Pharmaceutical Engineering
Depositing User:	Atroridho Rizky
Date Deposited:	04 Nov 2020 03:46
Last Modified:	04 Nov 2020 03:46
URI:	http://repository.sgu.ac.id/id/eprint/1607

Actions (login required)

View Item