Audah, Kholis Abdurachim Ultrasound-Assisted Extraction as Efficient Method for Obtaining Optimum Antioxidant from Mangrove Leaves of Rhizophora Mucronata. ULTRASOUND-ASSISTED EXTRACTION AS EFFICIENT METHOD FOR OBTAINING OPTIMUM ANTIOXIDANT FROM MANGROVE LEAVES OF RHIZOPHORA MUCRONATA.
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Abstract
Extraction method plays a critical role in the study of medicinal plants. Nowadays, a wide range of technologies is available to promote different methods of extraction with the better extraction output. The aim of the study was conducted to compare two extraction method, maceration and ultrasound-assisted extraction (UAE), for obtaining optimum antioxidant. The mangrove leaves of Rhizophora mucronata was chosen as a natural source of antioxidant. The leaves were extracted by employing 24 hours maceration and UAE in five different extraction times (5, 15, 30, 45 and 60 minutes). The experiments were conducted using water and 70% ethanol as solvents. The total phenolic content was determined by Folin-Ciocalteau assay and flavonoid content by Aluminium chloride assay. While the antioxidant activity was determined by the DPPH method. As a result, the phenolic compounds yielded by UAE with ethanol was considerably higher and need shorter time than maceration. The IC50 value was obtained by UAE with ethanol is 52.86 ppm. All these data showed that UAE with ethanol was the efficient method and mangrove leaves Rhizophora mucronata is a very potent natural antioxidant source.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS160 Plant extracts |
| Divisions: | Faculty of Life Sciences and Technology > Department of Biomedical Engineering |
| Depositing User: | Kholis Audah Abdurachim |
| Date Deposited: | 10 Apr 2023 01:40 |
| Last Modified: | 14 Apr 2023 03:07 |
| URI: | http://repository.sgu.ac.id/id/eprint/2478 |
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