Audah, Kholis Abdurachim Anticancer Screening of Mangrove Extract Library: Accelerating Drug Discovery from Indonesian Biodiversity. Anticancer Screening of Mangrove Extract Library: Accelerating Drug Discovery from Indonesian Biodiversity. (Unpublished)
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Abstract
Cancer is still one of the leading death causes in the world. There will be an estimated 18.1 million new cancer cases and over 9 million cancer deaths in 2018. Furthermore, some studies reported that new types of cancer had been discovered. Therefore, it is very important to find different sources for anticancer agents. Indonesian biodiversity is abundant natural resources that can be utilized as potential drug sources. Mangroves are among potential plant medicine that grow nearly at all Indonesian coastlines. The aim of this study was to evaluate the potential of mangrove extracts (extract library) as anticancer agents. In this study, eight mangroves species were used. There were 16 samples collected from different parts of the plants such as leaf, bark or root. Four types of solvents with different polarity were used producing 64 extracts. Brine Shrimp Lethality Test (BSLT) and MTT Assay were conducted for anticancer screening of mangrove extract library. There were 43 extracts showed anticancer potential with LC50 < 1000 ppm and 21 extracts with LC50 >1000 ppm. Next, 16 out of 43 extracts which have LC50< 500 ppm and some extracts with LC50 >1000 ppm were selected to be evaluated in MTT Cytotoxicity assay. There were 3 extracts that highly inhibited HeLa and MCF-7 Cell line. The highest inhibition percentage was recorded for ethyl acetate extract of root of Avicennia Marina (85 Ea), although it was not toxic enough in BSLT due to the high LC50 value (>1000 ppm). The next highest inhibition percentage were recorded for water extract of leaves of Sonneratia alba (88 A) and ethyl acetate extract of leaves of Xylocarpus granatum (86 Ea). In addition, there were 2 extracts that only effective to inhibit HeLa cell line, namely ethanol extract of leaves Bruguiera gymnorrhiza (75 Et) and ethyl acetate extract of root of Rhizophora apiculata (74 Ea). Phytochemical analysis of the extracts was also evaluated. The majority of samples showed saponin and tannin in considerable amount. This supported the data that mangrove extracts were potential as anticancer agents.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS160 Plant extracts |
| Divisions: | Faculty of Life Sciences and Technology > Department of Biomedical Engineering |
| Depositing User: | Kholis Audah Abdurachim |
| Date Deposited: | 12 Apr 2023 02:45 |
| Last Modified: | 14 Apr 2023 01:53 |
| URI: | http://repository.sgu.ac.id/id/eprint/2533 |
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