Molecular binding study of tetra-hydroxyl-ethyl-disulphate-2-sodium (thes) as a non-resistant antibacterial agent

Jogia, Gabriella Eldreen and Audah, Kholis A and Assiddiq, Bobby Fachrizal (2017) Molecular binding study of tetra-hydroxyl-ethyl-disulphate-2-sodium (thes) as a non-resistant antibacterial agent. Bachelor thesis, Swiss German University.

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Abstract

"Development on novel therapeutic agents and drugs is important to combat multidrug resistant bacterial infections. This study investigates molecular mechanism of Tetra-hydroxyl-ethyl-disulphate-2-sodium (THES) as a non-resistant bacterial agent with bactericidal properties against Gram-positive and Gram-negative bacteria. Isothermal titration calorimetry (ITC) technique was utilized to measure properties of binding between bacterial peptidoglycan and THES agent. The measurements were designed to verify the proposed mechanism of THES, which attack the peptidoglycan components to induce cell lysis and death. The exothermic model obtained suggested the presence of binding, verifying the proposed mechanism with dissociation value of 17.5 M. The study also provides visual evidence of THES mechanism on bacterial wall. Three-dimensional and cross-section measurement taken using atomic force microscope (AFM) showed deformation in the topography of bacteria after being treated with THES agent.

Item Type: Thesis (Bachelor)
Uncontrolled Keywords: Antibacterial ; Non-resistant agent ; Peptidoglycan-binding agent ; Multidrug-resistant ; Isothermal Titration Calorimetry
Subjects: R Medicine > R Medicine (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Life Sciences and Technology > Department of Biomedical Engineering
Depositing User: Adityatama Ratangga
Date Deposited: 29 Apr 2020 13:13
Last Modified: 29 Apr 2020 13:41
URI: http://repository.sgu.ac.id/id/eprint/51

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