Marsha, Talitha and Audah, Kholis Abdurachim and Bardant, Teuku Bauna (2017) Method development for analysis of antibacterial activity of thes and its derivative product against bacteria. Bachelor thesis, Swiss German University.
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Abstract
"The emergence of bacterial resistance has created the necessity for studies directed towards the development of new antibacterial agent. However, the antibacterial agents that have been available contained a lot of chemical additives which is harmful for human body. In response, Tetra Hidroxy Ethyl di Sulphate (THES) is now introduced. THES is a sulphate chelating agent trusted to have very low toxicity and have “non-resistant” characteristic as they have unique killing mechanism to fight against bacteria only by binding to the bacteria’s peptidoglycan layer. The aim of this study was to prove the antibacterial properties of THES and to select the best method for evaluating the antibacterial activity of THES and its product against bacteria. In this study, two variants of the agar diffusion method (well and disk), quantitative method established by OECD, and macrodilution assay were employed. Based on the result, the well-variant of the diffusion method was more preferable than the disk-variant. However, the macrodilution technique provided more suitable conditions for testing the antibacterial activity of THES and for determining the minimal inhibitory concentration. Quantitative method established by OECD was found to be excellent for proving the quality of product treated with THES and untreated product.
Item Type: | Thesis (Bachelor) |
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Uncontrolled Keywords: | Antibacterial activity ; Bacterial resistance ; A sulphate chelating agent ; THES |
Subjects: | R Medicine > R Medicine (General) |
Divisions: | Faculty of Life Sciences and Technology > Department of Biomedical Engineering |
Depositing User: | Adityatama Ratangga |
Date Deposited: | 29 Apr 2020 13:01 |
Last Modified: | 10 Feb 2022 03:56 |
URI: | http://repository.sgu.ac.id/id/eprint/54 |
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