Prasetyo, Dimas and Pandjaitan, Maruli and Santoso, Filiana (2018) Cytotoxicity Of Annona Muricata Extract With Addition Of Allium Sativa, Curcuma Mangga, And Bromelain For Breast And Liver Cancer Treatments. Bachelor thesis, Swiss German University.

Preview	Text Dimas Prasetyo 11405010 TOC.pdf Download (243kB) | Preview
	Text Dimas Prasetyo 11405010 1.pdf Restricted to Registered users only Download (176kB)
	Text Dimas Prasetyo 11405010 2.pdf Restricted to Registered users only Download (232kB)
	Text Dimas Prasetyo 11405010 3.pdf Restricted to Registered users only Download (398kB)
	Text Dimas Prasetyo 11405010 4.pdf Restricted to Registered users only Download (342kB)
	Text Dimas Prasetyo 11405010 5.pdf Restricted to Registered users only Download (147kB)
Preview	Text Dimas Prasetyo 11405010 Ref.pdf Download (198kB) | Preview

Abstract

Soursop leaves, garlic, and turmeric extract were known to have anticancer properties. A mixture of the 3 extracts (GST) has shown to have an even higher anticancer activity according to past studies. This study aims to utilize bromelain, an enzyme found in pineapple which has shown to be cytotoxic towards cancer cells to further increase the anticancer activity of the GST extract mixture. The analysis was done using NADH oxidase assay, brine shrimp lethality assay and MTT assay. The analysis of NADH oxidase inhibition rate was done using enzymatic reactions and the addition of bromelain increased the inhibition rate of the GST extract mixture from 41.244% up to 54.378%. The results of the MTT Assay against T47D breast cancer cells showed that the addition of bromelain could increase the anticancer activity of the GST extract mixture by 17.85%, from IC50 value of 32.45 ppm to 27.535 ppm and the activity increases in relation to the amount of bromelain added. MTT assay was also tested against HepG2 liver cancer cells but showed significantly lower potency even with increased amounts of bromelain. Lastly, brine shrimp lethality assay showed that the addition of bromelain could increase the cytotoxicity of the extracts by 28.38%.

Item Type:	Thesis (Bachelor)
Uncontrolled Keywords:	Annona Muricata; Allium Sativa; Curcuma Mangga; Bromelain; Anticancer
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RS Pharmacy and materia medica > RS160 Plant extracts
Divisions:	Faculty of Life Sciences and Technology > Department of Food Technology
Depositing User:	Astuti Kusumaningrum
Date Deposited:	27 May 2020 15:23
Last Modified:	27 May 2020 15:23
URI:	http://repository.sgu.ac.id/id/eprint/653

Actions (login required)

View Item