Harta, Rebecca and Puteri, Maria Dewi P. T. Gunawan and Wijaya, C. Hanny (2018) Antinutritional Factors Of Tempeh And Overripe Tempeh Made With Glucono Delta-Lactone Accelerated Acidification. Bachelor thesis, Swiss German University.

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Abstract

Tempeh-making process were known to reduce antinutritional factors in its original soybean. However, the effect of further fermentation in overripe tempeh as well as elimination of lactic acid fermentation step in GDL-accelerated acidification in the process are yet to be evaluated. In this study, the effect of both overriping process and GDL acidification was evaluated towards antinutritional factors, which include phytic acid, trypsin inhibitor, tannins and saponins. Phytic acid and trypsin inhibitor analyses were done using enzymatic reactions. Tannins analysis was conducted with the aid of HPLC, while analysis of saponins was conducted with qualitative foam formation method. The absence of natural lactic acid fermentation and shorter mold fermentation in GDL acidified tempeh processing does not affect antinutritional factors reduction. The impacts of overriping process in the reduction of antinutritional factors were only observed in phytic acid and saponins. Overriping process was shown to reduce phytic acid content from 1.072 g/100g to 0.774 g/100g in the application of natural acidification, and from 1.253 g/100g to 0.850 g/100g in the application of GDL acidification. Although it was already found that fermentation was able to reduce saponin content proportionally to the length of fermentation, this is the first study to show that overriping process was able to eliminate saponin content.

Item Type:	Thesis (Bachelor)
Uncontrolled Keywords:	GDL Acidification; Antinutritional Factors; Overripe Tempeh
Subjects:	Q Science > QP Physiology > QP141 Nutrition T Technology > TX Home economics > TX558 Tempeh
Divisions:	Faculty of Life Sciences and Technology > Department of Food Technology
Depositing User:	Astuti Kusumaningrum
Date Deposited:	30 May 2020 09:19
Last Modified:	30 May 2020 09:19
URI:	http://repository.sgu.ac.id/id/eprint/664

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