Study of Extraction and Stability Condition for Antioxidant Properties from Kelakai (Stenochlaenapalustris) Extract

Amanda, Nadia and Rahmawati, Della and Marpaung, Abdullah Muzi (2017) Study of Extraction and Stability Condition for Antioxidant Properties from Kelakai (Stenochlaenapalustris) Extract. Bachelor thesis, Swiss German University.

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Abstract

"The aims of this research are to discover the suitable conditions in the extraction and stability test for antioxidant activity from kelakai (Stenochlaena palustris) plant. The extraction process was done through two different solvents (ethanol & distilled water) and three extraction times (12, 24, 48 hours). It was found that samples which were extracted in distilled water for 48 hours had higher antioxidant activity, higher phenolic and flavonoids content. Next, the selected optimum extract was tested for its stability in different pH (4, 5, 6, 7), temperatures (5oC, 30oC, 50oC, 70oC, 90oC), and light conditions (bright and dark). From stability test with the effect of different pH, it was revealed that samples with addition of pH 4 and 5 had higher antioxidant activity. Likewise, samples with pH 5 were found to have higher phenolic and flavonoids content. As the effect of different temperatures, it was discovered that temperature of 70oC was suitable for antioxidant activity and flavonoids content of Stenochlaena palustris extract and 50oC was suitable for the phenolic content. All of the data within this research were obtained through analysis methods of DPPH scavenging activity, total phenolic content, and total flavonoids content assay. Some active components in optimum Stenochlaena palustris extract were also found through LC/MS analysis.

Item Type: Thesis (Bachelor)
Uncontrolled Keywords: Antioxidant Activity ; Stability ; Extraction ; Phenolic ; Flavonoids
Subjects: T Technology > TP Chemical technology > TP368 Food processing and manufacture
Divisions: Faculty of Life Sciences and Technology > Department of Food Technology
Depositing User: Adityatama Ratangga
Date Deposited: 14 May 2020 04:56
Last Modified: 14 May 2020 04:56
URI: http://repository.sgu.ac.id/id/eprint/84

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