Tirtakusuma, Ricky Fong and Pamungkas, Joko (2011) Development of Mesenchymal Stem Cells (MSCs) From Dental Pulp of Macaca Fascicularis. Bachelor thesis, Swiss German University.

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Abstract

The dental pulp of Macaca fascicularis was isolated and cultured to develop Mesenchymal Stem Cells (MSCs). The morphology of fibroblast-like cells and protein surface markers, including CD44, CD73, CD90, and CD105 were used to determine the characteristics of MSCs. Markers tests were conducted using RT-PCR. Afterwards, the dental pulp stem cells (DPSCs) were cultured in certain differentiation media to differentiate them into osteocytes, chondrocytes, and adipocytes. The research showed that morphologically all of those differentiations were formed. In addition, hematopoietic characteristics were also tested by CD34 and CD45 surface markers which were negatively expressed. Furthermore, pluripotent ability of stem cells also analyzed by Nanog, Oct4, and Sox2 markers which were negatively expressed too. Ultimately, the DPSCs of Macaca fascicularis as a non-human primate might be used as a model of human diseases.

Item Type:	Thesis (Bachelor)
Uncontrolled Keywords:	Mesenchymal Stem Cells; Dental Pulp Stem Cells; Macaca Fascicularis; Protein Surface Markers; Growth Factors
Subjects:	Q Science > QH Natural history > QH588 Stem cells R Medicine > R Medicine (General) > R856 Biomedical engineering
Divisions:	Faculty of Life Sciences and Technology > Department of Biomedical Engineering
Depositing User:	Astuti Kusumaningrum
Date Deposited:	04 Mar 2021 10:19
Last Modified:	04 Mar 2021 10:19
URI:	http://repository.sgu.ac.id/id/eprint/1051

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