Pricillia, Amanda and Pandjaitan, Maruli and Marpaung, Abdullah Muzi (2015) Cytotoxicity Assay of Saponin Containing Extract from Soursop Leaf Against Breast Cancer Cell. Bachelor thesis, Swiss German University.

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Abstract

This research was done to observe the activity of saponin contained in soursop leaf against breast cancer cell. The experiment has been done to determine the amount of saponin compound and its percentage of purity in soursop leaves extract. The extraction process was done by reflux extraction using 75% ethanol for 4 hours, followed by liquid extraction. The extraction was done in 2 different temperatures, o o namely 70 C and 90 C. Through the experiment it is found that temperature has no significant effect in the yield of saponin, however it has a huge contribute in influencing the percentage of purity. Percentage purity of extract which was extracted o using the temperature of 90 C is 38.785% with an average percentage yield 2.01%, which is equivalent to 0.1005 grams of total saponin content from 5 grams powdered soursop leaves. LC-MS analysis detected the presence of saponin at the retention time 1.96, with a name of Holothurinoside A, a non-sulfated saponin with a molecular formula C60H96O29 and molecular weight of 1280. In Brine Shrimp Lethality Test, the LC50 value of 10.16 ppm was obtained, and from the MTT assay, the extract containing saponin has an IC50 value of 60.103, which indicate that the extract is highly active and has a potent bioactivity.

Item Type:	Thesis (Bachelor)
Uncontrolled Keywords:	Annona Muricata; Saponin; Lethal Concentration; Cytotoxic Assay.
Subjects:	R Medicine > RS Pharmacy and materia medica > RS160 Plant extracts T Technology > TP Chemical technology > TP368 Food processing and manufacture
Divisions:	Faculty of Life Sciences and Technology > Department of Food Technology
Depositing User:	Atroridho Rizky
Date Deposited:	17 Dec 2020 17:12
Last Modified:	17 Dec 2020 17:12
URI:	http://repository.sgu.ac.id/id/eprint/1603

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