Priscilla, May and Pandjaitan, Maruli and Marpaung, Abdullah Muzi (2015) Cytotoxicity Assay of Saponin Containing Extract from Butterfly Pea Roots Against Breast Cancer Cell. Bachelor thesis, Swiss German University.

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Abstract

The research investigated the amount of total saponin content that can be obtained from varied extraction temperature of butterfly pea roots. The extraction temperature o o was varied to 70 C and 90 C. Through several extraction processes, it was proven that difference in extraction temperature did not give significant difference in total saponin content obtained. However, different extraction temperature gave significant difference in purity level of saponin in the extract. Extraction at 70˚C was proven to gave higher purity level of saponin and chosen as extraction temperature for the primary research. The average total saponin content acquired from extraction of 5 o grams powdered butterfly pea roots at 70 C was 0.808%, which is equivalent to 0.0404 grams. LC-MS analysis detected the presence of saponin compound named floratheasaponin A (C59H92O26) with molecular weight of 1217.38 g/mol. In Brine Shrimp Lethality Test (BSLT) test, the LC50 value of the sample was 17.53 ppm (<1000 ppm), which indicated the saponin containing extract is a promising plant- derived antitumor agents. According to MTT assay, the IC50 value of the extract towards breast cancer cell (T47D) was 32.184 ppm (<100 ppm), which means the saponin containing extract is a potential growth inhibitor of T47D (breast cancer cell line).

Item Type:	Thesis (Bachelor)
Uncontrolled Keywords:	Clitoria Ternatea; Saponin; Cytotoxicity Assay; Lethal concentration; Inhibition Concentration
Subjects:	Q Science > QD Chemistry > QD325 Saponins Q Science > QR Microbiology > QR185.5 Antibody-dependent cell cytotoxicity R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS160 Plant extracts T Technology > TP Chemical technology > TP368 Food processing and manufacture
Divisions:	Faculty of Life Sciences and Technology > Department of Food Technology
Depositing User:	Atroridho Rizky
Date Deposited:	28 Dec 2020 16:09
Last Modified:	28 Dec 2020 16:09
URI:	http://repository.sgu.ac.id/id/eprint/1648

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