Serafin, Livia and Kartawiria, Irvan S. and Abimanyu, Haznan (2017) Kinetic Studies And The Effect Of Substrate Concentration And Stirring Speed On Enzymatic Hydrolysis Of Cellulose From Pretreated Corn Cob. Bachelor thesis, Swiss German University.

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Abstract

Corn cob is one of lignocellulosic material for bioethanol production due to the high amount of cellulose, which can be converted into glucose through enzymatic hydrolysis and later be fermented into bioethanol. Unfortunately, the hydrolysis process is very time consuming and produce little amount of yield. Therefore, it is necessary to create a more efficient process by applying the optimum conditions including substrate concentration and stirring speed. The objective of this research is to study the effects of substrate concentration and stirring speed towards the amount of glucose produced, obtain optimum substrate concentration and stirring speed, and obtain kinetic model for the enzymatic hydrolysis process of corn cobs. The higher the substrate concentration, the higher amount of glucose will be produced. The higher the stirring speed will also result in higher production of glucose. However, if the stirring speed is too high, the enzymes is sensitive to mechanical stress and reduce activity at high speed. The optimum substrate concentration and stirring speed for enzymatic hydrolysis of corn cobs were found to be 15% (w/v) and 150 rpm respectively. The kinetic model used for the enzymatic hydrolysis of corn cobs is adopted from Yi Zhouliang and Eric Barson (2011) model, which shows the value of Vmax = 0.0014 mg/ml.h and KM= 0.32 mg/ml.

Item Type:	Thesis (Bachelor)
Uncontrolled Keywords:	Bioethanol; Enzymatic Hydrolysis; Lignocellulosic Biomass; Corn Cob; Stirring Speed
Subjects:	S Agriculture > SB Plant culture > SB183 Field crops T Technology > TP Chemical technology > TP248 Starch
Divisions:	Faculty of Life Sciences and Technology > Department of Pharmaceutical Engineering
Depositing User:	Astuti Kusumaningrum
Date Deposited:	18 May 2020 15:21
Last Modified:	18 May 2020 15:21
URI:	http://repository.sgu.ac.id/id/eprint/462

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