Manuella, Katya and Audah, Kholis Abdurachim and Sutanto, Hery (2016) Evaluation Of Antioxidant Activity Of Mangrove Tree Rhizophora Mucronata Leaf Extract. Bachelor thesis, Swiss German University.

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Abstract

Previous studies had reported R. mucronata extract as potential antioxidant agent. In this study, the antioxidant activity of R. mucronataleaf extract under different extraction conditions was evaluted. The study involved extracting the mangrove leaves using ultrasound-assisted extraction (UAE)under five different extraction times (5 min, 15 min, 30 min, 45 min, and 60 min), using distilled water and 70% ethanol as solvents. The extracts obtained through UAE were compared to the extract obtained through 24 hrs maceration. Folin-Ciocalteau assay was performed to determine the total phenolic content of the extracts, while diphenylpicryl-hydrazyl (DPPH) assay was performed for the assessment of antioxidant activity. Flavonoid content of the extracts was also measured. UAE extracts yielded high phenolic content (> 99.05 mg gallic acid equivalent/g dry mass raw material). Using 5 min extraction time, UAE produced comparable (ethanol extract) or better (aquaeous) polyphenol yield when compared to 24 hrs maceration. Strong correlation between total phenolic content and antioxidant activity was observed. While both aquaeous and ethanol extract displayed inhibitory activity towards DPPH, ethanol extract obtained through 5 min UAE displayed the highest antioxidant potential with IC50 of 52.85 mg dry mass raw material/L.

Item Type:	Thesis (Bachelor)
Uncontrolled Keywords:	Rhizophora Mucronata; Mangrove; Ultrasound Assisted Extraction; Antioxidant Activity; DPPH Scavenging Activity
Subjects:	Q Science > QK Botany > QK898 Anthocyanins Q Science > QK Botany > QK938 Mangrove plants R Medicine > R Medicine (General) > R856 Biomedical engineering R Medicine > RS Pharmacy and materia medica > RS160 Plant extracts
Divisions:	Faculty of Life Sciences and Technology > Department of Biomedical Engineering
Depositing User:	Astuti Kusumaningrum
Date Deposited:	29 Sep 2020 03:01
Last Modified:	29 Sep 2020 03:01
URI:	http://repository.sgu.ac.id/id/eprint/878

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