Kristianto, Hans and Pandjaitan, Maruli and Nugraha, Tutun (2008) Purification of High-Grade Papain from Latex of Carica Papaya. Bachelor thesis, Swiss German University.

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Abstract

High quality papain, especially high in enzyme activity is always in great demand by many industries such as pharmaceutical, food and beverage industries worldwide. However, some of the extraction processes damage the activity. In order to keep the enzyme activity, some processes that related to heat should be avoided. High temperature will damage the enzyme and causes it to denaturize. Hence, freeze dry was applied in the final drying process following the extraction stage. The process applied has yielded good results. At the end of the process, the specific activity of the enzyme was 148 u/mg protein. By avoiding heat process and some other processes that can cause the enzyme to denaturize, the experiment has produced high enzyme activity of papain.

Item Type:	Thesis (Bachelor)
Subjects:	Q Science > QP Physiology > QP601 Vitamin C > QP601.1 Papain T Technology > TD Environmental technology. Sanitary engineering > TD477 Water--Purification--Distillation process
Divisions:	Faculty of Life Sciences and Technology > Department of Pharmaceutical Engineering
Depositing User:	Astuti Kusumaningrum
Date Deposited:	02 Nov 2020 07:26
Last Modified:	02 Nov 2020 07:26
URI:	http://repository.sgu.ac.id/id/eprint/975

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