Marcellino, Rachel Amanda and Sutanto, Hery and Anita, Yulia (2015) Synthesis of Vanillin Derivatives Using Peroxidase Enzyme from Cauliflower. Bachelor thesis, Swiss German University.

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Abstract

Vanillin is widely known as a flavoring agent due to its phenolic structure, since phenol is categorized as a volatile organic compound. The hydroxyl group of vanillin has a potential to act as an antioxidant. From the synthesis process using vanillin, it was expected to synthesize a new compound that contains more hydroxyl groups in order to increase the antioxidant activity value of vanillin. This synthesis process was assisted by peroxidase enzyme obtained from cauliflower as the catalyst and hydrogen peroxide as an oxidizing agent. However, using cauliflower as the source of peroxidase enzyme has not been used in much research. Peroxidase catalyzed the oxidation process by decomposing the hydrogen peroxide and reacted with the substrate that was being converted into a radical substrate. The synthesized product was suspected to be dihydroquercetin or taxifolin, the LCMS spectrum supported the facts related to taxifolin. Taxifolin was found to be a strong antioxidant due to its phenol hydroxyl group. Unfortunately, the antioxidant activity of taxifolin obtained from this experiment was low compared to the antioxidant activity of vanillin. Thus, this result was not consistent to many journals that study about the antioxidant activity of taxifolin. To ensure the structure of taxifolin, FTIR and NMR analysis should be done in the next research.

Item Type:	Thesis (Bachelor)
Uncontrolled Keywords:	Synthesis; Vanillin; Vanillin Derivatives; Taxifolin; Peroxidase Enzyme; Antioxidant Activity.
Subjects:	R Medicine > RS Pharmacy and materia medica > RS160 Plant extracts T Technology > TP Chemical technology T Technology > TP Chemical technology > TP368 Food processing and manufacture
Divisions:	Faculty of Life Sciences and Technology > Department of Food Technology
Depositing User:	Atroridho Rizky
Date Deposited:	29 Dec 2020 14:54
Last Modified:	29 Dec 2020 14:54
URI:	http://repository.sgu.ac.id/id/eprint/1653

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