Synthesis Of Ferulic Acid Derivatives Using Peroxidase Enzyme

Melina, Raissa and Sutanto, Hery and Anita, Yulia (2016) Synthesis Of Ferulic Acid Derivatives Using Peroxidase Enzyme. Bachelor thesis, Swiss German University.

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Abstract

Ferulic acid is widely known as antioxidant due to the hydroxyl group in its phenolic structure. The synthesis reaction was done in order to increase the antioxidant activity. It was expected to synthesize a new compound that contains more hydroxyl group. Initially, ferulic acid was esterified to obtain maximum result of synthesis product. Esterification product was tested using GCMS. The synthesis reaction was then assisted by peroxidase enzyme as the catalyst. Synthesis of ferulic acid derivatives using peroxidase enzyme is still limited. Peroxidase enzyme catalyzed the oxidation process by breaking down hydrogen peroxide and covert the substrate into a radical molecule. The synthesis product was suspected as tetraethyl ferulate. The LCMS spectrum supports the facts related to tetraethyl ferulate formation. Tetraethyl ferulate was found to be strong antioxidant due to its structure. The antioxidant of ferulic acid, ethyl ferulate and tetraethyl ferulate was tested against DPPH. The antioxidant activity of tetraethyl ferulate in this experiment was the highest compared to the others. In the next research, the synthesis product is recommended to be purified and analyzed further about the structure using FTIR and NMR.

Item Type: Thesis (Bachelor)
Uncontrolled Keywords: Synthesis; Ferulic Acid; Ferulic Acid Derivatives; Tetraethyl Ferulate; Antioxidant Activity
Subjects: Q Science > QK Botany > QK898 Anthocyanins
Q Science > QP Physiology > QP752 Fatty acids in human nutrition
T Technology > TP Chemical technology > TP368 Food processing and manufacture
Divisions: Faculty of Life Sciences and Technology > Department of Food Technology
Depositing User: Astuti Kusumaningrum
Date Deposited: 09 Nov 2020 11:47
Last Modified: 09 Nov 2020 11:47
URI: http://repository.sgu.ac.id/id/eprint/903

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